Lin Chiao-Ying, Hovhannisyan Vladimir, Wu June-Tai, Lin Chii-Wann, Chen Jyh-Horng, Lin Sung-Jan, Dong Chen-Yuan
National Taiwan University, Department of Electrical Engineering, Taipei, Taiwan.
J Biomed Opt. 2008 Sep-Oct;13(5):050502. doi: 10.1117/1.2981817.
The fruit fly Drosophila melanogaster is one of the most valuable organisms in studying genetics and developmental biology. To gain insight into Drosophila development, we successfully acquired label-free, in vivo images of both developing muscles and internal organs in a stage 2 larva using the minimally invasive imaging modality of multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy. We found that although MAF is useful in identifying structures such as the digestive system, trachea, and intestinal track, it is the SHG signal that allowed the investigation of the muscular architecture within the developing larva. Our results suggest that multiphoton microscopy is a powerful in vivo, label-free imaging technique to examine Drosophila physiology and may be used for developmental studies.
果蝇黑腹果蝇是研究遗传学和发育生物学最有价值的生物之一。为了深入了解果蝇的发育过程，我们使用多光子自发荧光（MAF）和二次谐波产生（SHG）显微镜的微创成像方式，成功获取了2龄幼虫发育中的肌肉和内部器官的无标记体内图像。我们发现，虽然MAF在识别消化系统、气管和肠道等结构方面很有用，但正是SHG信号使得我们能够研究发育中幼虫的肌肉结构。我们的结果表明，多光子显微镜是一种强大的用于检查果蝇生理学的体内无标记成像技术，可用于发育研究。
