Wu Qinfeng, Kumar Nilay, Velagala Vijay, Zartman Jeremiah J
Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556 USA.
J Biol Eng. 2019 Apr 23;13:33. doi: 10.1186/s13036-019-0161-8. eCollection 2019.
Reverse-engineering how complex multicellular systems develop and function is a grand challenge for systems bioengineers. This challenge has motivated the creation of a suite of bioengineering tools to develop increasingly quantitative descriptions of multicellular systems. Here, we survey a selection of these tools including microfluidic devices, imaging and computer vision techniques. We provide a selected overview of the emerging cross-talk between engineering methods and quantitative investigations within developmental biology. In particular, the review highlights selected recent examples from the system, an excellent platform for understanding the interplay between genetics and biophysics. In sum, the integrative approaches that combine multiple advances in these fields are increasingly necessary to enable a deeper understanding of how to analyze both natural and synthetic multicellular systems.
逆向工程复杂多细胞系统的发育和功能对系统生物工程师来说是一项重大挑战。这一挑战推动了一系列生物工程工具的创建，以对多细胞系统进行越来越定量的描述。在这里，我们概述了其中一些工具，包括微流控设备、成像和计算机视觉技术。我们对工程方法与发育生物学中的定量研究之间新兴的相互作用进行了精选综述。特别是，该综述重点介绍了来自该系统的一些近期实例，该系统是理解遗传学和生物物理学之间相互作用的绝佳平台。总之，结合这些领域的多项进展的综合方法对于更深入地理解如何分析天然和合成多细胞系统越来越必要。
