Denton Michael L, Foltz Michael S, Schuster Kurt J, Noojin Gary D, Estlack Larry E, Thomas Robert J
Northrop Grumman, Warfighter Concepts and Applications Department, San Antonio, Texas, USA.
J Biomed Opt. 2008 Sep-Oct;13(5):054014. doi: 10.1117/1.2981831.
Without effective in vitro damage models, advances in our understanding of the physics and biology of laser-tissue interaction would be hampered due to cost and ethical limitations placed on the use of nonhuman primates. We extend our characterization of laser-induced cell death in an existing in vitro retinal model to include damage thresholds at 514 and 413 nm. The new data, when combined with data previously reported for 532 and 458 nm exposures, provide a sufficiently broad range of wavelengths and exposure durations (0.1 to 100 s) to make comparisons with minimum visible lesion (in vivo) data in the literature. Based on similarities between in vivo and in vitro action spectra and temporal action profiles, the cell culture model is found to respond to laser irradiation in a fundamentally similar fashion as the retina of the rhesus animal model. We further show that this response depends on the amount of intracellular melanin pigmentation.
由于在使用非人类灵长类动物方面存在成本和伦理限制，若没有有效的体外损伤模型，我们对激光与组织相互作用的物理和生物学的理解进展将会受到阻碍。我们将现有体外视网膜模型中激光诱导细胞死亡的特征描述扩展至包括514和413nm处的损伤阈值。这些新数据与先前报道的532和458nm曝光数据相结合，提供了足够宽的波长范围和曝光持续时间（0.1至100秒），以便与文献中的最小可见损伤（体内）数据进行比较。基于体内和体外作用光谱以及时间作用曲线之间的相似性，发现细胞培养模型对激光照射的反应与恒河猴动物模型的视网膜在根本上相似。我们进一步表明，这种反应取决于细胞内黑色素的色素沉着量。
