Zhuo Shuangmu, Chen Jianxin, Yu Biying, Jiang Xingshan, Luo Teng, Liu Quangang, Chen Rong, Xie Shusen
Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fuzhou 350007, P. R. China.
J Biomed Opt. 2008 Sep-Oct;13(5):054024. doi: 10.1117/1.2982534.
Because of frequent exposure to carcinogens, the bronchus is prone to early pathologic alterations. The assessment of these early changes is of key significance in physiological studies and disease diagnosis of the bronchus. We utilize nonlinear optical microscopy (NLOM) to image mouse bronchial tissue based on intrinsic nonlinear optical contrast. Our results show that NLOM is effective for imaging the bronchial intact microstructural components, providing quantitative information about the biomorphology and biochemistry of tissue. Our findings also display that NLOM can provide a two-photon ratiometric redox fluorometry, based on mitochondrial signals and reduced pyridine nucleotide (NADH and NADPH) and oxidized flavoproteins (Fp) signals, to assess the metabolic state of the epithelial cells and chondrocytes. It was found that NLOM can offer a sensitive tool, based on the second-harmonic signal depth-dependent decay, to obtain quantitative information on the optical property of the stroma associated with normal and diseased tissue states. Our results suggest that with the advent of the clinical portability of typical nonlinear optical endoscopy, the NLOM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of bronchial disease.
由于频繁接触致癌物，支气管容易出现早期病理改变。评估这些早期变化在支气管的生理学研究和疾病诊断中具有关键意义。我们利用非线性光学显微镜（NLOM）基于内在非线性光学对比度对小鼠支气管组织进行成像。我们的结果表明，NLOM对于成像支气管完整的微观结构成分是有效的，可提供有关组织生物形态学和生物化学的定量信息。我们的研究结果还显示，NLOM可以基于线粒体信号以及还原型吡啶核苷酸（NADH和NADPH）和氧化型黄素蛋白（Fp）信号提供双光子比率氧化还原荧光测定法，以评估上皮细胞和软骨细胞的代谢状态。研究发现，基于二次谐波信号深度依赖性衰减，NLOM可以提供一种灵敏的工具，以获取与正常和患病组织状态相关的基质光学性质的定量信息。我们的结果表明，随着典型非线性光学内窥镜临床便携性的出现，NLOM技术有潜力在体内应用于支气管疾病的临床诊断和监测。
