Gharekhan Anita H, Arora Siddharth, Mayya K B K, Panigrahi Prasanta K, Sureshkumar M B, Pradhan Asima
Gujarat University, C. U. Shah Science College, Ahmedabad, 380 009, India.
J Biomed Opt. 2008 Sep-Oct;13(5):054063. doi: 10.1117/1.2997376.
We study the spectral correlation properties of the polarized fluorescence spectra of normal and cancerous human breast tissues, corresponding to patients belonging to diverse age groups and socioeconomic backgrounds. The emission range in the visible wavelength regime of 500 to 700 nm is analyzed, with the excitation wavelength at 488 nm, where flavin is one of the active fluorophores. The correlation matrices for parallel and perpendicularly polarized fluorescence spectra reveal correlated domains, differing significantly in normal and cancerous tissues. These domains can be ascribed to different fluorophores and absorbers in the tissue medium. The spectral fluctuations in the perpendicular component of the cancerous tissue clearly reveal randomization not present in the normal channel. Random matrix-based predictions for the spectral correlations match quite well with the observed behavior. The eigenvectors of the correlation matrices corresponding to large eigenvalues clearly separate out different tissue types and identify the dominant wavelengths, which are active in cancerous tissues.
我们研究了正常和癌性人类乳腺组织的偏振荧光光谱的光谱相关特性，这些组织对应于不同年龄组和社会经济背景的患者。分析了500至700nm可见波长范围内的发射范围，激发波长为488nm，其中黄素是活性荧光团之一。平行和垂直偏振荧光光谱的相关矩阵揭示了相关域，在正常组织和癌性组织中有显著差异。这些域可归因于组织介质中的不同荧光团和吸收体。癌性组织垂直分量中的光谱波动清楚地显示出正常通道中不存在的随机化。基于随机矩阵的光谱相关性预测与观察到的行为相当吻合。对应于大特征值的相关矩阵的特征向量清楚地分离出不同的组织类型，并识别出在癌性组织中起作用的主导波长。
