Hamblin C, Mellor P S, Graham S D, Hooghuis H, Montejano R C, Cubillo M A, Boned J
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey.
Epidemiol Infect. 1991 Apr;106(2):365-71. doi: 10.1017/s0950268800048512.
A total of 256 sera collected from three species of domesticated equidae in four different Spanish provinces were examined 1-4 months after the administration of attenuated monovalent African horse sickness virus (AHSV) serotype 4 vaccine. Approximately 10% of the sera were negative by ELISA, virus neutralization, agar gel immuno-diffusion and complement fixation tests. Similar negative reactions were recorded with sera from two ponies after experimental primary vaccination. The rapid rise in antibodies in sera from these two ponies, after a second dose of vaccine, suggested they would probably have been immune to challenge. It is therefore suggested that the apparent absence of antibodies against AHSV in some animals after primary vaccination may not necessarily indicate a total lack of protection.
在西班牙四个不同省份，对三种家养马科动物接种单价减毒非洲马瘟病毒4型（AHSV-4）疫苗1至4个月后采集的256份血清进行了检测。通过酶联免疫吸附测定（ELISA）、病毒中和试验、琼脂凝胶免疫扩散试验和补体结合试验，约10%的血清呈阴性。实验性初次接种疫苗后，两匹小马的血清也出现了类似的阴性反应。这两匹小马在接种第二剂疫苗后，血清中抗体迅速上升，表明它们可能对病毒攻击具有免疫力。因此，有人认为初次接种疫苗后，某些动物体内看似没有抗AHSV抗体，不一定意味着完全缺乏保护作用。
