Hamblin C, Graham S D, Anderson E C, Crowther J R
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey.
Epidemiol Infect. 1990 Apr;104(2):303-12. doi: 10.1017/s0950268800059483.
A competition enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid identification and quantification of antibodies against African horse sickness (AHS) in sera from solipeds. The data showed the ELISA to be sensitive, specific and reliable. More than 1600 sera from 37 different countries were examined and results compared with those obtained by agar gel immuno-diffusion (AGID) tests. In no case did any of 775 sera from countries where AHS has never been reported and where AHS vaccines are not used, record an ELISA titre greater than 4. A titre equal to or greater than 8 was considered positive. Using this criterion, 96.3% of sera tested in both assays were in agreement. Doubtful results by AGID (1.7%) were clearly defined in terms of positivity and negativity by ELISA. This ELISA is suited for the rapid laboratory confirmation of AHS and should be considered as a replacement for the traditional AGID test.
已开发出一种竞争性酶联免疫吸附测定（ELISA）法，用于快速鉴定和定量单蹄兽血清中抗非洲马瘟（AHS）的抗体。数据表明该ELISA法灵敏、特异且可靠。检测了来自37个不同国家的1600多份血清，并将结果与琼脂凝胶免疫扩散（AGID）试验所得结果进行比较。在从未报告过AHS且未使用AHS疫苗的国家的775份血清中，无一例ELISA滴度超过4。滴度等于或大于8被视为阳性。采用该标准，两种检测方法检测的血清中有96.3%结果一致。AGID检测的可疑结果（1.7%）通过ELISA明确界定为阳性或阴性。这种ELISA适用于AHS的快速实验室确诊，应被视为传统AGID检测的替代方法。
