Ribeiro Dimas M, Desikan Radhika, Bright Jo, Confraria Ana, Harrison Judith, Hancock John T, Barros Raimundo S, Neill Steven J, Wilson Ian D
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Minas Gerais, Brazil.
Plant Cell Environ. 2009 Jan;32(1):46-57. doi: 10.1111/j.1365-3040.2008.01906.x. Epub 2008 Nov 10.
Abscisic acid (ABA)-induced stomatal closure is mediated by a complex, guard cell signalling network involving nitric oxide (NO) as a key intermediate. However, there is a lack of information concerning the role of NO in the ABA-enhanced stomatal closure seen in dehydrated plants. The data herein demonstrate that, while nitrate reductase (NR)1-mediated NO generation is required for the ABA-induced closure of stomata in turgid leaves, it is not required for ABA-enhanced stomatal closure under conditions leading to rapid dehydration. The results also show that NO signalling in the guard cells of turgid leaves requires the ABA-signalling pathway to be both capable of function and active. The alignment of this NO signalling with guard cell Ca(2+)-dependent/independent ABA signalling is discussed. The data also highlight a physiological role for NO signalling in turgid leaves and show that stomatal closure during the light-to-dark transition requires NR1-mediated NO generation and signalling.
脱落酸（ABA）诱导的气孔关闭是由一个复杂的保卫细胞信号网络介导的，其中一氧化氮（NO）作为关键中间体。然而，关于NO在脱水植物中ABA增强的气孔关闭中的作用，目前还缺乏相关信息。本文数据表明，虽然在膨压叶片中，硝酸盐还原酶（NR）1介导的NO生成是ABA诱导气孔关闭所必需的，但在导致快速脱水的条件下，ABA增强的气孔关闭并不需要它。结果还表明，膨压叶片保卫细胞中的NO信号需要ABA信号通路既能发挥功能又处于活跃状态。本文讨论了这种NO信号与保卫细胞Ca(2+)依赖性/非依赖性ABA信号的一致性。数据还突出了NO信号在膨压叶片中的生理作用，并表明光暗转换期间的气孔关闭需要NR1介导的NO生成和信号传递。
