Nautiyal C S, Dion P, Chilton W S
Département de Phytologie, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Ste-Foy, Québec, Canada.
J Bacteriol. 1991 May;173(9):2833-41. doi: 10.1128/jb.173.9.2833-2841.1991.
The characteristics of mannopine and mannopinic acid utilization by Agrobacterium tumefaciens B6S3, Arthrobacter sp. strain MBA209, and Pseudomonas putida NA513 were studied. Strain B6S3 utilized the four mannityl opines, mannopine, mannopinic acid, agropine, and agropinic acid. It also utilized several mannityl opine analogs, which were modified in either the sugar or the amino acid moiety. It utilized mannopine more rapidly after preincubation on mannopine, mannopinic acid, or glutamine than after pregrowth on glucose, mannose, or mannitol. Strains MBA209 and NA513 utilized mannopine and mannopinic acid, but not the other two mannityl opines. They utilized few mannityl opine analogs, sometimes because of failure to utilize the products of initial cleavage of the analog. Utilization of mannopine and mannopinic acid by strain NA513 was strictly dependent on prior growth on these substrates. A spontaneous regulatory variant of strain NA513 remained unable to utilize most of the mannityl opine analogs. Glutamine, mannose, and several analogs had no inhibitory effect on [14C]mannopine utilization by strain NA513.
研究了根癌土壤杆菌B6S3、节杆菌属菌株MBA209和恶臭假单胞菌NA513利用甘露碱和甘露碱酸的特性。菌株B6S3利用四种甘露糖型冠瘿碱，即甘露碱、甘露碱酸、农杆碱和农杆碱酸。它还利用了几种甘露糖型冠瘿碱类似物，这些类似物在糖或氨基酸部分有所修饰。与在葡萄糖、甘露糖或甘露醇上预培养后相比，在甘露碱、甘露碱酸或谷氨酰胺上预培养后，它利用甘露碱的速度更快。菌株MBA209和NA513利用甘露碱和甘露碱酸，但不利用其他两种甘露糖型冠瘿碱。它们利用的甘露糖型冠瘿碱类似物很少，有时是因为无法利用类似物初始裂解的产物。菌株NA513对甘露碱和甘露碱酸的利用严格依赖于在这些底物上的前期生长。菌株NA513的一个自发调节变体仍然无法利用大多数甘露糖型冠瘿碱类似物。谷氨酰胺、甘露糖和几种类似物对菌株NA513利用[14C]甘露碱没有抑制作用。
