Unilateral intra-perilymphatic infusion of substance P enhances ipsilateral vestibulo-ocular reflex gains in the sinusoidal rotation test.

Previous studies have reported localization of substance P (SP) within the inner ear and that SP exists abundantly within vestibular endorgans. While SP's functional role in the inner ear remains unclear, SP can act as a neuromodulator in the CNS and directly influences neuronal excitability. We hypothesized that SP might influence neuronal excitability within the vestibular periphery. The present study used the sinusoidal rotation test to investigate the influence of SP after its local application in the guinea pig unilateral inner ear. A tiny hole was made adjacent to the round window in the right ears of Hartley white guinea pigs that had normal tympanic membranes and Preyer reflexes. An osmotic pump infused SP (10(-4)M, 10(-3)M, and 10(-2)M), neurokinin-1 (NK-1) receptor antagonist (10(-3)M) alone, or SP (10(-3)M)+NK-1 receptor antagonist (10(-3)M) through this hole, with rotation tests performed before, and 12h and 24h after the treatment. Results were used to calculate the vestibulo-ocular reflex (VOR) gains. After administration of 10(-3)M and 10(-2)M SP, significant increases in the VOR gains were noted at 12h after treatment, with these gains disappearing by 24h after treatment. This increase was not observed when there was simultaneous NK-1 receptor antagonist administration. There were also no changes in the VOR gains noted after administration of 10(-4)M SP or the NK-1 receptor antagonist alone. These results indicate the possibility that SP may act on vestibular endorgans as an excitatory factor via the NK-1 receptors.