Miles R J, Taylor R R, Varsani H
Microbial Physiology Research Group, King's College, London.
J Med Microbiol. 1991 Apr;34(4):219-23. doi: 10.1099/00222615-34-4-219.
Oxygen uptake and H2O2 accumulation during the metabolism of glucose and glycerol by whole cells, and of L-alpha-glycerophosphate (GP) and NADH by cells lysed with Triton, was determined for the type strains of six fermentative Mycoplasma species. Oxidation of glucose and of NADH by M. mycoides, M. pneumoniae and M. putrefaciens was accompanied by the accumulation of relatively small quantities of H2O2 (less than 0.05 mol/mol O2), though larger quantities (0.17-0.24 mol/mol O2) were produced by M. dispar. M. fermentans and M. canis were distinguished from the other strains used in that O2 uptake in the presence of glucose could not be demonstrated. However, metabolism of glucose was indicated by a reduction in the pH of the suspending medium and lysed cells oxidised NADH with the production of approximately 1.0 mol H2O2/mol O2 taken up. Glycerol was oxidised by all the strains studied except M. fermentans, and large quantities of H2O2 (0.48-1.07 mol/mol O2) accumulated. Cells of the glycerol-oxidising strains, lysed with Triton, oxidised GP with the production of approximately 1.0 mol H2O2/mol O2 utilised, which indicated the presence of a GP oxidase. The importance of H2O2 production as a factor in the pathogenicity of some mycoplasmas might depend upon the availability of glycerol in vivo.
测定了六种发酵型支原体菌株的全细胞在葡萄糖和甘油代谢过程中的氧气摄取及过氧化氢积累情况，以及经 Triton 裂解的细胞在 L-α-甘油磷酸酯（GP）和 NADH 代谢过程中的氧气摄取及过氧化氢积累情况。丝状支原体、肺炎支原体和腐败支原体对葡萄糖和 NADH 的氧化伴随着相对少量过氧化氢的积累（小于 0.05 mol/mol O₂），而差异支原体产生的过氧化氢量较大（0.17 - 0.24 mol/mol O₂）。发酵支原体和犬支原体与其他所用菌株的区别在于，在葡萄糖存在的情况下无法证明有氧气摄取。然而，悬浮培养基 pH 值的降低表明了葡萄糖的代谢，裂解细胞氧化 NADH 时，每摄取 1 mol O₂会产生约 1.0 mol 过氧化氢。除发酵支原体外，所有研究的菌株都能氧化甘油，且积累了大量过氧化氢（0.48 - 1.07 mol/mol O₂）。经 Triton 裂解的甘油氧化菌株的细胞氧化 GP 时，每利用 1 mol O₂会产生约 1.0 mol 过氧化氢，这表明存在 GP 氧化酶。过氧化氢产生作为某些支原体致病性的一个因素，其重要性可能取决于体内甘油的可用性。
