Drew David, Klepsch Mirjam M, Newstead Simon, Flaig Ralf, De Gier Jan-Willem, Iwata So, Beis Konstantinos
Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London, UK.
Mol Membr Biol. 2008 Dec;25(8):677-82. doi: 10.1080/09687680802552257.
Gastrointestinal bacteria, like Escherichia coli, must remove bile acid to survive in the gut. Bile acid removal in E. coli is thought to be mediated primarily by the multidrug efflux pump, AcrB. Here, we present the structure of E. coli AcrB in complex with deoxycholate at 3.85 A resolution. All evidence suggests that bile acid is transported out of the cell via the periplasmic vestibule of the AcrAB-TolC complex.
胃肠道细菌，如大肠杆菌，必须去除胆汁酸才能在肠道中生存。大肠杆菌中的胆汁酸去除被认为主要由多药外排泵AcrB介导。在这里，我们展示了分辨率为3.85埃的大肠杆菌AcrB与脱氧胆酸盐复合物的结构。所有证据表明，胆汁酸通过AcrAB-TolC复合物的周质前庭被转运出细胞。
