Franzellitti Silvia, Valbonesi Paola, Contin Andrea, Biondi Carla, Fabbri Elena
Interdepartmental Centre for Environmental Science Research, University of Bologna, 48100 Ravenna, Italy.
Radiat Res. 2008 Oct;170(4):488-97. doi: 10.1667/rr1405.1.
The heat-shock proteins (HSPs) are important cellular stress markers and have been proposed as candidates to infer biological effects of high-frequency electromagnetic fields (EMFs). In the current study, HSP70 gene and protein expression were evaluated in cells of the human trophoblast cell line HTR-8/SVneo after prolonged exposure (4 to 24 h) to 1.8 GHz continuous-wave (CW) and different GSM signals (GSM-217Hz and GSM-Talk) to assess the possible effects of time and modulation schemes on cell responses. Inducible HSP70 protein expression was not modified by high-frequency EMFs under any condition tested. The inducible HSP70A, HSP70B and the constitutive HSC70 transcripts did not change in cells exposed to high-frequency EMFs with the different modulation schemes. Instead, levels of the inducible HSP70C transcript were significantly enhanced after 24 h exposure to GSM-217Hz signals and reduced after 4 and 16 h exposure to GSM-Talk signals. As in other cell systems, in HTR-8/SVneo cells the response to high-frequency EMFs was detected at the mRNA level after exposure to amplitude-modulated GSM signals. The present results suggest that the expression analysis for multiple transcripts, though encoding the same or similar protein products, can be highly informative and may account for subtle changes not detected at the protein level.
热休克蛋白（HSPs）是重要的细胞应激标志物，已被提议作为推断高频电磁场（EMFs）生物学效应的候选指标。在本研究中，对人滋养层细胞系HTR-8/SVneo的细胞进行长时间（4至24小时）暴露于1.8 GHz连续波（CW）和不同的GSM信号（GSM-217Hz和GSM-Talk）后，评估热休克蛋白70（HSP70）基因和蛋白表达，以评估时间和调制方式对细胞反应的可能影响。在任何测试条件下，高频电磁场均未改变可诱导的HSP70蛋白表达。在暴露于不同调制方式的高频电磁场的细胞中，可诱导的HSP70A、HSP70B和组成型热休克同源蛋白70（HSC70）转录本没有变化。相反，在暴露于GSM-217Hz信号24小时后，可诱导的HSP70C转录本水平显著升高，而在暴露于GSM-Talk信号4小时和16小时后降低。与其他细胞系统一样，在HTR-8/SVneo细胞中，暴露于调幅GSM信号后，在mRNA水平检测到对高频电磁场的反应。目前的结果表明，对多种转录本的表达分析，尽管编码相同或相似的蛋白质产物，但可能具有高度的信息性，并可能解释在蛋白质水平未检测到的细微变化。
