Zhou D, White R H
Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.
J Bacteriol. 1991 May;173(10):3250-1. doi: 10.1128/jb.173.10.3250-3251.1991.
The transfer of sulfur from methionine to cysteine in the archaebacteria Sulfolobus acidocaldarius and Halobacterium marismortui was studied by feeding 34S-labeled methionine to cells and measuring the incorporation of 34S into protein-bound cellular cysteine and methionine by mass spectrometry. It was found that, as are eucaryotes, both of these archaebacteria were able to convert the sulfur of methionine to cysteine.
通过向嗜热栖热菌和死海嗜盐菌的细胞中加入34S标记的甲硫氨酸,并利用质谱法测量34S掺入与蛋白质结合的细胞半胱氨酸和甲硫氨酸中的情况,研究了古细菌嗜热栖热菌和死海嗜盐菌中甲硫氨酸的硫向半胱氨酸的转移。结果发现,与真核生物一样,这两种古细菌都能够将甲硫氨酸的硫转化为半胱氨酸。