非产甲烷古细菌中叶酸的分析与表征
Analysis and characterization of the folates in the nonmethanogenic archaebacteria.
作者信息
White R H
机构信息
Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg 24061.
出版信息
J Bacteriol. 1988 Oct;170(10):4608-12. doi: 10.1128/jb.170.10.4608-4612.1988.
A detailed analysis of the folate coenzymes in the nonmethanogenic archaebacteria has been performed. By using the Lactobacillus casei microbiological assay for folates, the levels of folates in Sulfolobus solfataricus and Sulfolobus acidocaldarius were found to be 3.7 and 8.3 ng/g (dry weight) of cells, respectively, compared with 88,000 and 28,000 ng/g (dry weight) of cells in Halobacterium halobium and Halobacterium strain GN-1, respectively. The levels of folates found in the Sulfolobus spp. were approximately 100 times less than those found in the typical eubacterium, whereas the levels in the halobacteria were approximately 10 times higher. The folate in Sulfolobus solfataricus was shown to consist of only 5-formyltetrahydropteroylglutamate, and the folate in Halobacterium strain GN-1 was shown to consist of only pteroyldiglutamate. The low folate levels in the Sulfolobus spp. are the same as those found in the methanogenic bacteria, suggesting that another C1 carrier may function in these cells.
已对非产甲烷古细菌中的叶酸辅酶进行了详细分析。通过使用干酪乳杆菌微生物法测定叶酸，发现嗜热栖热菌和嗜酸热硫化叶菌中的叶酸水平分别为3.7和8.3 ng/g（干重）细胞，相比之下，嗜盐菌和GN-1菌株中的叶酸水平分别为88,000和28,000 ng/g（干重）细胞。在硫化叶菌属中发现的叶酸水平比典型真细菌中的叶酸水平低约100倍，而在嗜盐菌中的叶酸水平则高约10倍。嗜热栖热菌中的叶酸仅由5-甲酰四氢蝶酰谷氨酸组成，而GN-1菌株中的叶酸仅由蝶酰二谷氨酸组成。硫化叶菌属中的叶酸水平较低，与产甲烷细菌中的叶酸水平相同，这表明另一种C1载体可能在这些细胞中发挥作用。
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