Kelsey M I, Sexton S A
J Chromatogr. 1977 Mar 21;133(2):327-34. doi: 10.1016/s0021-9673(00)83490-1.
Lithocholic acid metabolites produced by the intestinal microflora of rats can be isolated from other endogenous lipids using Sephadex LH-20 column chromatography. Analyses of individual metabolites collected from the column by silica gel coated glass fiber paper chromatography result in the resolution of epimeric 3-hydroxy derivatives. In addition, glass fiber paper chromatography is more sensitive and requires less development time than conventional glass-coated thin-layer plates. Further confirmation of the identity of metabolites is achieved by gas-liquid chromatography, which separates both methyl and ethyl esters of lithocholic and isolithocholic acids.
大鼠肠道微生物群产生的石胆酸代谢物可通过葡聚糖凝胶LH - 20柱色谱法从其他内源性脂质中分离出来。通过硅胶涂层玻璃纤维纸色谱法对从柱上收集的单个代谢物进行分析，可分离出差向异构的3 - 羟基衍生物。此外，玻璃纤维纸色谱法比传统的玻璃涂层薄板更灵敏，展开时间更短。通过气液色谱法进一步确认代谢物的身份，该方法可分离石胆酸和异石胆酸的甲酯和乙酯。
