硫酸化和非硫酸化石胆酸及其甘氨酸和牛磺酸共轭物的薄层色谱分离
Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugates.
作者信息
Cass O W, Cowen A E, Hofmann A F, Coffin S B
出版信息
J Lipid Res. 1975 Mar;16(2):159-60.
PMID:1127355
Abstract
A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic acid containing calcium sulfate (10% by weight) under conditions of chamber saturation.
摘要
描述了一种用于高效薄层色谱分离石胆酸及其N-甘氨酸和N-牛磺酸共轭物，以及它们各自的3α-硫酸盐的方法。使用氯仿-甲醇-乙酸-水65:24:15:9（v/v）的溶剂系统，在腔室饱和条件下，于含有硫酸钙（重量百分比为10%）的硅胶空气干燥板上进行分离。
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