Alfaqeh H, Chua K H, Aminuddin B S, Ruszymah B H I
Department of Physiology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
Med J Malaysia. 2008 Jul;63 Suppl A:119-20.
This study aimed to compare the effects of three different media on the in vivo chondrogenesis of sheep bone marrow stem cells (BMSC). Sheep BMSC were cultured in F12:DMEM + 10% FBS, chondrogenic medium containing 5ng/ml TGF,3 + 50ng/ml IGF-1 and UKM-MECC for three weeks. The cultured cells were then harvested for construct formation with fibrin. Constructed tissues were implanted subcutaneously into nude mice for in vivo development. Cell aggregates were formed in both chondrogenic medium and UKM-MECC demonstrated the early chondrogenesis process. After five weeks of in vivo development, both chondrogenic medium and UKM-MECC promoted cartilage matrix synthesis confirmed by Safranin O staining.
本研究旨在比较三种不同培养基对绵羊骨髓干细胞（BMSC）体内软骨形成的影响。将绵羊BMSC在F12:DMEM + 10%胎牛血清、含5ng/ml转化生长因子β3 + 50ng/ml胰岛素样生长因子-1的软骨形成培养基以及UKM-MECC中培养三周。然后收获培养的细胞，用纤维蛋白构建组织。将构建的组织皮下植入裸鼠体内进行体内发育。在软骨形成培养基和UKM-MECC中均形成了细胞聚集体，证明了早期软骨形成过程。经过五周的体内发育，软骨形成培养基和UKM-MECC均促进了软骨基质合成，番红O染色证实了这一点。
