Alfaqeh H, Norhamdan M Y, Chua K H, Chen H C, Aminuddin B S, Ruszymah B H I
Department of Physiology, UKM Medical Centre, Universiti Kebangsaan Malaysia.
Med J Malaysia. 2008 Jul;63 Suppl A:37-8.
This study was to determine if autologous bone marrow mesenchymal stem cells (BMSCs) cultured in chondrogenic medium could repair surgically induced osteoarthritis. Sheep BMSCs were cultured in medium containing 5ng/ml TGFbeta3 + 50ng/ml IGF-1 for three weeks. The cultured cells were then suspended at density of 2x10(6) cell/ml and injected intraarticularly into the osteoarthritic knee joint. After six weeks, the distal head of the femur and the proximal tibial plateau were removed and stained with H&E. The results indicated that knee joints treated with autologous BMSCs cultured in chondrogenic medium showed clear evidence of articular cartilage regeneration in comparison with other groups.
本研究旨在确定在软骨形成培养基中培养的自体骨髓间充质干细胞（BMSCs）是否能修复手术诱导的骨关节炎。将绵羊BMSCs在含有5ng/ml转化生长因子β3（TGFβ3）+50ng/ml胰岛素样生长因子-1（IGF-1）的培养基中培养三周。然后将培养的细胞以2×10⁶细胞/ml的密度悬浮，并关节腔内注射到骨关节炎膝关节中。六周后，取出股骨远端头部和胫骨近端平台并用苏木精和伊红（H&E）染色。结果表明，与其他组相比，用在软骨形成培养基中培养的自体BMSCs治疗的膝关节显示出关节软骨再生的明显证据。
