Fickers Patrick, Destain Jacqueline, Thonart Philippe
Centre Wallon de Biologie Industrielle, Service de Technologie Microbienne, Université de Liège, Boulevard du Rectorat, Batiment 40, Liège, Belgium.
J Basic Microbiol. 2009 Apr;49(2):212-5. doi: 10.1002/jobm.200800186.
Two different types of fed-batch fermentation were investigated to improve production yields of the Lip2 extracellular lipase in Y. lipolytica mutant-strain LgX64.81 grown in a 20l bioreactor. Compare to batch cultures, culture feeding with the complete medium led to a 2-fold increased lipase production (2016 +/- 76 U ml(-1)) whereas addition of a combination of glucose and olive oil led to a 3-fold increase. The high level of lipase production obtained on glucose media with Y. lipolytica LgX64.81 could be related to its phenotype, i.e., a lower sensibility to glucose catabolite repression due to a modification in the level of HXK1 expression.
为提高解脂耶氏酵母突变株LgX64.81在20升生物反应器中生长时Lip2胞外脂肪酶的产量，研究了两种不同类型的补料分批发酵。与分批培养相比，用完全培养基进行补料培养使脂肪酶产量提高了2倍（2016±76 U ml(-1)），而添加葡萄糖和橄榄油的组合则使产量提高了3倍。在葡萄糖培养基上利用解脂耶氏酵母LgX64.81获得的高水平脂肪酶产量可能与其表型有关，即由于HXK1表达水平的改变，对葡萄糖分解代谢物阻遏的敏感性较低。
