Vandermies Marie, Fickers Patrick
TERRA Teaching and Research Centre, Microbial Processes and Interactions, University of Liège⁻Gembloux AgroBio Tech, 5030 Gembloux, Belgium.
Microorganisms. 2019 Jan 30;7(2):40. doi: 10.3390/microorganisms7020040.
Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possibility of post-translational modifications. is considered as one of the most attractive hosts due to its ability to metabolize raw substrate, to express genes at a high level, and to secrete protein in large amounts. In recent years, several reviews have been dedicated to genetic tools developed for this purpose. Though the construction of efficient cell factories for recombinant protein synthesis is important, the development of an efficient process for recombinant protein production in a bioreactor constitutes an equally vital aspect. Indeed, a sports car cannot drive fast on a gravel road. The aim of this review is to provide a comprehensive snapshot of process tools to consider for recombinant protein production in bioreactor using as a cell factory, in order to facilitate the decision-making for future strain and process engineering.
重组蛋白生产代表着一个价值数十亿美元的市场。因此，它在学术界和工业界都是一个重要的研究领域。使用酵母作为细胞工厂具有若干优势，例如易于进行基因操作、能够在高细胞密度下生长以及具有进行翻译后修饰的可能性。由于其具有代谢原始底物、高水平表达基因以及大量分泌蛋白质的能力，酵母被认为是最具吸引力的宿主之一。近年来，已有多篇综述专门介绍了为此目的开发的遗传工具。尽管构建用于重组蛋白合成的高效细胞工厂很重要，但开发一种在生物反应器中生产重组蛋白的高效工艺同样至关重要。事实上，一辆跑车在砾石路上无法快速行驶。本综述的目的是全面概述在使用酵母作为细胞工厂的生物反应器中进行重组蛋白生产时可考虑的工艺工具，以便为未来的菌株和工艺工程决策提供便利。
