Do Diem T Hoang, Theron Chrispian W, Fickers Patrick
Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux AgroBio Tech, Av. de la Faculté, 2B. B-5030 Gembloux, Belgium.
Microorganisms. 2019 Jul 31;7(8):229. doi: 10.3390/microorganisms7080229.
Non-conventional yeasts are efficient cell factories for the synthesis of value-added compounds such as recombinant proteins, intracellular metabolites, and/or metabolic by-products. Most bioprocess, however, are still designed to use pure, ideal sugars, especially glucose. In the quest for the development of more sustainable processes amid concerns over the future availability of resources for the ever-growing global population, the utilization of organic wastes or industrial by-products as feedstocks to support cell growth is a crucial approach. Indeed, vast amounts of industrial and commercial waste simultaneously represent an environmental burden and an important reservoir for recyclable or reusable material. These alternative feedstocks can provide microbial cell factories with the required metabolic building blocks and energy to synthesize value-added compounds, further representing a potential means of reduction of process costs as well. This review highlights recent strategies in this regard, encompassing knowledge on catabolic pathways and metabolic engineering solutions developed to endow cells with the required metabolic capabilities, and the connection of these to the synthesis of value-added compounds. This review focuses primarily, but not exclusively, on as a yeast cell factory, owing to its broad range of naturally metabolizable carbon sources, together with its popularity as a non-conventional yeast.
非常规酵母是合成重组蛋白、细胞内代谢物和/或代谢副产物等增值化合物的高效细胞工厂。然而，大多数生物过程仍设计用于使用纯净的理想糖类，尤其是葡萄糖。在日益增长的全球人口对未来资源可用性感到担忧的情况下，寻求开发更可持续的过程时，利用有机废物或工业副产品作为支持细胞生长的原料是一种关键方法。事实上，大量的工业和商业废物同时构成了环境负担，也是可回收或可再利用材料的重要储存库。这些替代原料可以为微生物细胞工厂提供合成增值化合物所需的代谢构件和能量，进一步代表了降低过程成本的潜在手段。本综述重点介绍了这方面的最新策略，包括关于分解代谢途径的知识以及为使细胞具备所需代谢能力而开发的代谢工程解决方案，以及这些与增值化合物合成的联系。由于其广泛的天然可代谢碳源以及作为非常规酵母的受欢迎程度，本综述主要但不限于将其作为酵母细胞工厂进行探讨。
