Kaur Gurvinder, Padmaja V
Department of Botany, Andhra University, Visakhapatnam, India.
J Basic Microbiol. 2009 Jun;49(3):264-74. doi: 10.1002/jobm.200800156.
Extracellular enzymes produced by Beauveria bassiana, are believed to play a key role in cuticle hydrolysis. Enzyme production and pathogenicity has been found to be positively correlated. Twenty-eight isolates of B. bassiana, collected from different geographical regions and host ranges were characterized by in vitro extracellular enzyme production and SDS-PAGE techniques for discerning biochemical basis for virulence among the different isolates. In vitro analysis of extracellular enzymes like protease, amylase, caseinase, chitinase and lipase was undertaken in an attempt to understand their relevance to virulence of the isolates. The different isolates of B. bassiana were evaluated for virulence to the second instar larvae of Helicoverpa armigera in laboratory bioassays. SDS-PAGE of total intracellular soluble proteins was also studied in order to understand affinities among the different isolates of B. bassiana. The relationship between enzyme production and pathogenicity and vice versa was nearly 50%. There was a 50% relationship associated with original insect host, pathogenicity and enzyme production.
球孢白僵菌产生的胞外酶被认为在角质层水解中起关键作用。已发现酶的产生与致病性呈正相关。从不同地理区域和寄主范围收集的28株球孢白僵菌，通过体外胞外酶产生和SDS-PAGE技术进行表征，以辨别不同分离株中毒力的生化基础。对蛋白酶、淀粉酶、酪蛋白酶、几丁质酶和脂肪酶等胞外酶进行体外分析，试图了解它们与分离株毒力的相关性。在实验室生物测定中评估了球孢白僵菌不同分离株对棉铃虫二龄幼虫的毒力。还研究了总细胞内可溶性蛋白的SDS-PAGE，以了解球孢白僵菌不同分离株之间的亲缘关系。酶产生与致病性之间的关系以及反之亦然的关系接近50%。与原始昆虫寄主、致病性和酶产生相关的关系为50%。
