Velavan Viswakethu, Dhanapal Rajendran, Ramkumar Govindaraju, Karthi Sengodan, Senthil-Nathan Sengottayan, Ndomba Osmund A, Kweka Eliningaya J
ICAR-National Bureau of Agricultural Insect Resources, Bengaluru 560024, India.
Ashoka Trust for Research in Ecology and the Environment (ATREE), Bengaluru 560064, India.
J Fungi (Basel). 2022 Jan 10;8(1):68. doi: 10.3390/jof8010068.
A field survey was done in teak ( F.) forests in South India to explore the entomopathogenic effect of (Ascomycota: Sordariomycetes) against teak defoliator, (Lepidoptera: Hyblaeidae). About 300 soils and infected insect samples were collected during the survey and thirty-six fungal isolates were isolated from soil and insect samples and characterized. The fungi were cultured on PDAY with dodine and antibiotics. Generally, the EPF culture was incubated at 27 °C in darkness for 15 days. Virulence of the Entomopathogenic Fungi (EPF) ability to germinate under cold and heat temperatures was assessed in a culture impregnated with conidia. In the experiment, it was found that for the first time , , and species caused significantly higher mortality to hosts. These isolates of , and were all considered to be effective virulent and environmentally adaptive. The isolates were recommended as effective bio-control agents through the field investigation of teak defoliator from South India forest. This study paves the way to utilize the indigenous isolates of EPF for the control of teak defoliator and to combat the pests thatare resistant to insecticide.
在印度南部的柚木（柚木属）森林中进行了一项实地调查，以探究子囊菌门粪壳菌纲的某种真菌对柚木叶蛾（鳞翅目：夜蛾科）的昆虫病原效应。调查期间收集了约300份土壤和受感染昆虫样本，从土壤和昆虫样本中分离出36株真菌菌株并进行了鉴定。这些真菌在添加了多果定和抗生素的PDAY培养基上培养。一般来说，昆虫病原真菌培养物在27℃黑暗条件下孵育15天。在含有分生孢子的培养物中评估了昆虫病原真菌（EPF）在冷热温度下的萌发能力及其毒力。在该实验中，首次发现某几个物种对宿主造成的死亡率显著更高。这些某几个物种的分离株均被认为具有有效的毒力且适应环境。通过对来自印度南部森林的柚木叶蛾进行实地调查，这些分离株被推荐为有效的生物防治剂。本研究为利用昆虫病原真菌的本土分离株来控制柚木叶蛾以及对抗对杀虫剂具有抗性的害虫铺平了道路。
