Riddihough G, Ish-Horowicz D
Imperial Cancer Research Fund, Department of Zoology, University of Oxford, UK.
Genes Dev. 1991 May;5(5):840-54. doi: 10.1101/gad.5.5.840.
Striped expression of the pair-rule gene hairy (h) plays a central role in regulating segmentation in Drosophila. We have used h-lacZ reporter gene fusions to delineate h sequences that drive individual stripe expression. We show that 14 kb of 5'-flanking DNA directs expression of seven lacZ stripes in the blastoderm embryo. Within this region, we identify discrete sequences required for expression of individual stripes 1, 5, 6, and 7, and dispersed elements active in the stripe 2 domain. Only the stripe 1 element directs lacZ expression in an accurate h stripe; stripes 5, 6, and 7 are displaced by one to two cells relative to their h counterparts. These results indicate that regulatory sequences are dispersed within the h promoter. We have determined the sensitivity of the lacZ stripes to maternal, gap, and pair-rule gene mutations. Our results suggest that different but overlapping subsets of gap genes regulate each stripe and that activation and repression are both important in generating the stripe pattern.
成对规则基因hairy（h）的条纹状表达在果蝇的体节调控中起着核心作用。我们利用h-lacZ报告基因融合来描绘驱动单个条纹表达的h序列。我们发现，14 kb的5'侧翼DNA指导胚盘胚中七个lacZ条纹的表达。在该区域内，我们鉴定出了条纹1、5、6和7表达所需的离散序列，以及在条纹2区域活跃的分散元件。只有条纹1元件能准确地指导lacZ在h条纹中的表达；条纹5、6和7相对于它们的h对应条纹会偏移一到两个细胞。这些结果表明调控序列分散在h启动子内。我们已经确定了lacZ条纹对母体、间隙和成对规则基因突变的敏感性。我们的结果表明，间隙基因的不同但重叠的子集调控每个条纹，并且激活和抑制在产生条纹模式中都很重要。
