Ribeiro Alice M, Bocca Anamelia L, Amaral André C, Faccioli Lucia H, Galetti Fabio C S, Zárate-Bladés Carlos R, Figueiredo Florencio, Silva Célio L, Felipe Maria Sueli S
Laboratory of Pathology, Faculty of Medicine, University of Brasília, Brasilia, DF 70910-900, Brazil.
Vaccine. 2009 Jan 22;27(4):606-13. doi: 10.1016/j.vaccine.2008.10.022. Epub 2008 Nov 24.
Heat-shock proteins are molecules with extensive data showing their potential as immunomodulators of different types of diseases. The gene of HSP65 from Mycobacterium leprae has shown prophylactic and immunotherapeutic effects against a broad arrays of experimental models including tuberculosis, leishmaniasis, arthritis and diabetes. With this in mind, we tested the DNAhsp65 vaccine using an experimental model of Paraccocidiodomycosis, an important endemic mycosis in Latin America. The intramuscular immunization with DNAhsp65 induced, in BALB/c mice, an increase of Th1-levels cytokines and a reduction of fungal burdens resulted in a marked reduction of collagen and lung remodeling. DNAhsp65 may be an attractive candidate for prevention, therapy and as an adjuvant for mycosis treatment.
热休克蛋白是一类分子，大量数据表明它们具有作为不同类型疾病免疫调节剂的潜力。来自麻风分枝杆菌的HSP65基因已在包括结核病、利什曼病、关节炎和糖尿病在内的一系列实验模型中显示出预防和免疫治疗作用。考虑到这一点，我们使用拉丁美洲一种重要的地方性真菌病——副球孢子菌病的实验模型对DNAhsp65疫苗进行了测试。在BALB/c小鼠中，用DNAhsp65进行肌肉内免疫诱导了Th1水平细胞因子的增加，真菌负荷的减少导致胶原蛋白和肺部重塑显著减少。DNAhsp65可能是预防、治疗以及作为真菌病治疗佐剂的有吸引力的候选物。
