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使用壳聚糖聚合物纳米颗粒包裹的P10肽的鼻内疫苗作为小鼠模型中副球孢子菌病的实验性治疗方法

Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model.

作者信息

Rodrigues Dos Santos Junior Samuel, Kelley Lopes da Silva Francenya, Santos Dias Lucas, Oliveira Souza Ana Camila, Valdemir de Araujo Marcelo, Buffoni Roque da Silva Leandro, Travassos Luiz R, Correa Amaral Andre, P Taborda Carlos

机构信息

Departamento de Microbiologia, Instituto de Ciências Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil.

Laboratorio de Nano & Biotecnologia, Departamento de Biotecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goias, Goiania, GO 74605-050, Brazil.

出版信息

J Fungi (Basel). 2020 Sep 2;6(3):160. doi: 10.3390/jof6030160.

DOI:10.3390/jof6030160
PMID:32887256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7560165/
Abstract

Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the dimorphic fungal species of , which mainly affects the lungs. Modern strategies for the treatment and/or prevention of PCM are based on a Th1-type immune response, which is important for controlling the disease. One of the most studied candidates for a vaccine is the P10 peptide, derived from the 43 kDa glycoprotein of . In order to improve its immune modulatory effect, the P10 peptide was associated with a chitosan-conjugated nanoparticle. The nanoparticles presented 220 nm medium size, poly dispersion index (PDI) below 0.5, zeta potential of +20 mV and encapsulation efficiency around 90%. The nanoparticles' non-toxicity was verified by hemolytic test and cell viability using murine macrophages. The nanoparticles were stable and presented physicochemical characteristics desirable for biological applications, reducing the fungal load and the usual standard concentration of the peptide from 4 to 20 times.

摘要

副球孢子菌病（PCM）是一种由双态真菌引起的肉芽肿性真菌疾病，主要影响肺部。现代治疗和/或预防PCM的策略基于Th1型免疫反应，这对控制疾病很重要。最受研究的疫苗候选物之一是源自43 kDa糖蛋白的P10肽。为了提高其免疫调节作用，P10肽与壳聚糖共轭纳米颗粒结合。纳米颗粒的平均尺寸为220 nm，多分散指数（PDI）低于0.5，zeta电位为+20 mV，包封效率约为90%。通过溶血试验和使用小鼠巨噬细胞的细胞活力验证了纳米颗粒的无毒性。纳米颗粒稳定，具有生物应用所需的物理化学特性，可将真菌负荷和肽的常规标准浓度降低4至20倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/445a995c58b1/jof-06-00160-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/1f002af9bc98/jof-06-00160-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/a4102dbe1deb/jof-06-00160-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/abaad24279df/jof-06-00160-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/132f782840d6/jof-06-00160-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/445a995c58b1/jof-06-00160-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/1f002af9bc98/jof-06-00160-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/a4102dbe1deb/jof-06-00160-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/abaad24279df/jof-06-00160-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/132f782840d6/jof-06-00160-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8fe/7560165/445a995c58b1/jof-06-00160-g005a.jpg

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