Onaran Ilhan, Tezcan Gülçin, Ozgönenel Levent, Cetin Esra, Ozdemir Alper Tunga, Kanigür-Sultuybek Gönül
Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.
Rheumatol Int. 2009 May;29(7):797-800. doi: 10.1007/s00296-008-0772-8. Epub 2008 Nov 22.
The findings of the studies on poly(ADP-ribose) polymerase-1 (PARP-1) have suggested that the enzyme inactivation provides significant protection against systemic or tissue inflammation in animal models. It has also shown that the single-nucleotide polymorphism (Val762Ala) of the PARP-1 causes about 40% decrease of enzyme activity. The aim of this study was to analyze the association of the PARP-1 Val762Ala polymorphism in Turkish patients with rheumatoid arthritis. A total of 128 RA patients and 165 normal controls from the same geographic region were studied and polymerase chain reaction (PCR)-based restriction analysis was used to identify Val762Ala polymorphism of the PARP-1. Association analyses were performed using chi (2) tests. Our results indicated that the distribution of the PARP-1 genotypes and alleles did not differ significantly among subjects with or without RA (P > 0.05). The results of the study indicate that, for our Turkish sample, the V762A polymorphism of the PARP-1 may not be involved in susceptibility to RA, implying that the polymorphism may not function as a candidate gene marker for screening RA patients.
关于聚（ADP - 核糖）聚合酶 -1（PARP -1）的研究结果表明，在动物模型中该酶的失活能为全身性或组织炎症提供显著保护。研究还表明，PARP -1的单核苷酸多态性（Val762Ala）会导致酶活性降低约40%。本研究的目的是分析土耳其类风湿性关节炎患者中PARP -1 Val762Ala多态性的相关性。共研究了来自同一地理区域的128例类风湿性关节炎患者和165例正常对照，采用基于聚合酶链反应（PCR）的限制性分析来鉴定PARP -1的Val762Ala多态性。使用卡方检验进行关联分析。我们的结果表明，PARP -1基因型和等位基因的分布在有或无类风湿性关节炎的受试者之间没有显著差异（P>0.05）。研究结果表明，对于我们的土耳其样本，PARP -1的V762A多态性可能与类风湿性关节炎的易感性无关，这意味着该多态性可能无法作为筛选类风湿性关节炎患者的候选基因标记。
