Department of Radiotherapy and Gynecological Oncology, Greater Poland Cancer Center Poznan, Poznan, Poland.
Mol Diagn Ther. 2013 Aug;17(4):239-45. doi: 10.1007/s40291-013-0036-5.
Data on the Val762Ala (rs1136410) polymorphism in the poly(adenosine diphosphate [ADP]-ribose) polymerase 1 (PARP-1) gene as a risk factor for various types of cancers in different ethnicities are inconsistent. We studied this association in a Caucasian population.
Using high-resolution melting curve analysis (HRM), we studied the distribution of the PARP-1 Val762Ala polymorphism in patients with cervical cancer (n = 446) and in controls (n = 491).
Logistic regression analysis adjusting for age, pregnancy, oral contraceptive use, tobacco smoking, and menopausal status demonstrated that the PARP-1 Val762Ala polymorphism was associated with an increased risk of cervical cancer. The adjusted odds ratio (OR) for patients with the Ala/Val genotype versus the Val/Val genotype was 1.381 (95 % CI = 1.025-1.859, p = 0.033), and the adjusted OR for the Ala/Ala or Ala/Val genotype versus the Val/Val genotype was 1.403 (95 % CI = 1.057-1.863, p = 0.019). The p value from the chi-square test of the trend observed for the PARP-1 Val762Ala polymorphism was statistically significant (p trend = 0.0123). Stratified analyses of the PARP-1 Val762Ala genotype distribution and cervical cancer risk showed that the age-adjusted OR of Ala/Ala or Ala/Val vs Val/Val for pregnancy was 1.388 (95 % CI = 1.027-1.877, p = 0.0328), 1.773 (95 % CI = 1.145-2.745, p = 0.0100) for contraceptive use, and 1.604 (95 % CI = 1.132-2.272, p = 0.0077) for postmenopausal women. The age-adjusted OR of Ala/Val vs Val/Val for contraceptive use was 1.769 (95 % CI = 1.114-2.809, p = 0.0154) and for postmenopausal women was 1.577 (95 % CI = 1.094-2.272, p = 0.0143).
Our studies suggest that the PARP-1 Val762Ala polymorphism may be a genetic risk factor for cervical cancer.
多聚（二磷酸腺苷-核糖）聚合酶 1（PARP-1）基因中的 Val762Ala（rs1136410）多态性作为不同种族各种类型癌症的风险因素的数据不一致。我们在白种人群中研究了这种相关性。
使用高分辨率熔解曲线分析（HRM），我们研究了宫颈癌患者（n=446）和对照组（n=491）中 PARP-1 Val762Ala 多态性的分布。
调整年龄、妊娠、口服避孕药使用、吸烟和绝经状态后的逻辑回归分析表明，PARP-1 Val762Ala 多态性与宫颈癌风险增加相关。与 Val/Val 基因型相比，Ala/Val 基因型患者的调整优势比（OR）为 1.381（95%CI=1.025-1.859，p=0.033），Ala/Ala 或 Ala/Val 基因型与 Val/Val 基因型相比的调整 OR 为 1.403（95%CI=1.057-1.863，p=0.019）。PARP-1 Val762Ala 多态性的趋势检验的卡方检验 p 值具有统计学意义（p趋势=0.0123）。PARP-1 Val762Ala 基因型分布与宫颈癌风险的分层分析表明，对于妊娠，Ala/Ala 或 Ala/Val 与 Val/Val 的年龄调整 OR 为 1.388（95%CI=1.027-1.877，p=0.0328），避孕药使用的 1.773（95%CI=1.145-2.745，p=0.0100）和绝经后妇女的 1.604（95%CI=1.132-2.272，p=0.0077）。避孕药使用的 Ala/Val 与 Val/Val 的年龄调整 OR 为 1.769（95%CI=1.114-2.809，p=0.0154），绝经后妇女为 1.577（95%CI=1.094-2.272，p=0.0143）。
我们的研究表明，PARP-1 Val762Ala 多态性可能是宫颈癌的遗传风险因素。
