高致病性禽流感病毒A/Anhui/1/2005（H5N1）感染的人肺癌细胞的差异表达
[The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 (H5N1)].
作者信息
Hu Xiao-Fen, Liu Qin-Zhi, Li Chuan, Dong Jie, Zhou Jian-Fang, Wang Min, Shu Yue-Long, Liu Hong-Tu, Liang Mi-Fang, Li De-Xin
机构信息
State Key Laboratory for Infectious Diseases, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Jun;22(3):180-2.
OBJECTIVE
To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1.
METHODS
The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology. The candidate genes were selected and confirmed by quantitative real-time PCR.
RESULTS
The spectrum of genes with the differential expression in the cells infected with H5N1 was obtained and 16 candidate genes were identified in the cellular apoptosis pathway, mTOR pathway, and the cellular immunity as well.
CONCLUSIONS
Our results suggest that H5N1 exert a stronger impact on eliciting apoptosis of infected cells than the common influenza virus H1N1.
目的
鉴定感染高致病性禽流感病毒H5N1的人类细胞中的基因。
方法
分别用H5N1和H1N1感染肺癌细胞系A549。在感染后的不同时间点收获感染的细胞，并通过微阵列技术筛选差异表达的基因。通过定量实时PCR选择并确认候选基因。
结果
获得了感染H5N1的细胞中差异表达基因的谱图，并在细胞凋亡途径、mTOR途径和细胞免疫中鉴定出16个候选基因。
结论
我们的结果表明，H5N1比普通流感病毒H1N1对诱导感染细胞凋亡的影响更强。
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