Feng Xiang-hui, Zhang Li, Meng Huan-xin, Xu Li, Chen Zhi-bin, Shi Dong
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Jul;43(7):402-5.
To investigate the prevalence of Actinobacillus actinomycetemcomitans (Aa) in whole saliva of different types of periodontitis and compare the detections of Aa between saliva and pooled subgingival plaque sample, and analyze the relationship between Aa and clinical conditions.
Unstimulated whole saliva samples and pooled subgingival samples were collected from 50 aggressive periodontitis (AgP) patients, 48 chronic periodontitis (CP) patients and 25 subjects with no periodontitis, and Aa was detected in these samples by PCR method.
The prevalence of Aa in whole saliva of AgP patients was significantly higher than in subjects with no periodontitis (32% vs. 4%, P<0.01) and CP patients (32% vs. 15%, P<0.05). Aa was also more frequently detected in whole saliva sample than in pooled subgingival sample of AgP patients (32% vs. 16%, P<0.05). Subjects younger than 30 years old were more likely to present Aa in whole saliva ( OR = 3.23, P<0.05) and percentage of sites with bleeding index (BI) > or = 3 over 70% was a risk indicator for the presence of Aa in whole saliva
The detection of Aa in whole saliva sample of AgP patients was more frequent than in pooled subgingival plaque samples, and also more frequent than in CP patients and subjects with no periodontitis, which suggest that Aa may participate in the initiation and progression of aggressive periodontitis.
调查不同类型牙周炎患者全唾液中伴放线放线杆菌（Aa）的流行情况，比较唾液与龈下菌斑混合样本中Aa的检出情况，并分析Aa与临床状况之间的关系。
收集50例侵袭性牙周炎（AgP）患者、48例慢性牙周炎（CP）患者和25例无牙周炎受试者的非刺激性全唾液样本和龈下菌斑混合样本，采用聚合酶链反应（PCR）法检测这些样本中的Aa。
AgP患者全唾液中Aa的流行率显著高于无牙周炎受试者（32%对4%，P<0.01）和CP患者（32%对15%，P<0.05）。AgP患者全唾液样本中Aa的检出频率也高于龈下菌斑混合样本（32%对16%，P<0.05）。年龄小于30岁的受试者全唾液中更易出现Aa（OR=3.23，P<0.05），出血指数（BI）≥3的部位占比超过70%是全唾液中存在Aa的风险指标。
AgP患者全唾液样本中Aa的检出频率高于龈下菌斑混合样本，也高于CP患者和无牙周炎受试者，这表明Aa可能参与侵袭性牙周炎的发生和发展。
