Department of Periodontology and Preventive Dentistry, Dental Research Division, University of Taubaté, Rua Expedicionário Ernesto Pereira, 110, Taubaté 12020-330, SP, Brazil.
Arch Oral Biol. 2010 Mar;55(3):242-8. doi: 10.1016/j.archoralbio.2010.01.008. Epub 2010 Feb 19.
Previous studies have suggested that Aggregatibacter actinomycetemcomitans is involved in the aetiology of aggressive periodontitis as well as chronic periodontitis. In addition, some authors have also reported that serotype-specific antigens of A. actinomycetemcomitans determine the severity of disease. This study aimed to elucidate the prevalence of A. actinomycetemcomitans and the distribution of A. actinomycetemcomitans serotypes in Brazilian subjects with chronic periodontitis.
A total of 486 individuals were enrolled in this survey. All patients received clinical examinations that included periodontal pocket depth, clinical attachment loss, plaque, and gingival indexes. Subgingival samples were taken for microbial analysis. The genomic DNA of A. actinomycetemcomitans was provided by PCR.
Out of 486 subjects examined, A. actinomycetemcomitans was isolated in 85 (17.5%) individuals. Out of 85 positive samples, 68 were infected by at least 1 serotype, 7 by mixed infection, and 10 were non-serotyped. Serotypes d and f were not detected. Serotype c showed the highest prevalence (52.9%), followed by serotype a (31.8%).
Intragroup analysis revealed that, in slight/moderate periodontitis, serotypes c and a were significantly more prevalent than serotypes b and d-f; the prevalence of serotype c in severe periodontitis was significantly greater than that of serotypes a and b. Our data were similar in Asian and Eurasian populations.
先前的研究表明，伴放线放线杆菌与侵袭性牙周炎和慢性牙周炎的发病机制有关。此外，一些作者还报告称，伴放线放线杆菌的血清型特异性抗原决定疾病的严重程度。本研究旨在阐明巴西慢性牙周炎患者中伴放线放线杆菌的流行情况以及伴放线放线杆菌血清型的分布。
本研究共纳入 486 名个体。所有患者均接受了临床检查，包括牙周袋深度、临床附着丧失、菌斑和牙龈指数。采集龈下样本进行微生物分析。采用 PCR 法提供伴放线放线杆菌的基因组 DNA。
在 486 名受检者中，85 名（17.5%）个体分离出伴放线放线杆菌。在 85 个阳性样本中，68 个样本感染了至少 1 个血清型，7 个样本为混合感染，10 个样本未定型。未检测到血清型 d 和 f。血清型 c 的流行率最高（52.9%），其次是血清型 a（31.8%）。
组内分析显示，在轻度/中度牙周炎中，血清型 c 和 a 的流行率明显高于血清型 b 和 d-f；在重度牙周炎中，血清型 c 的流行率明显高于血清型 a 和 b。我们的数据与亚洲和欧亚人群的数据相似。
