[大肠杆菌脂多糖对体外分化的人牙髓细胞矿化基质形成的影响]
[Effect of Escherichia coli lipopolysaccharide on mineralized matrix formation in vitro differentiation human dental pulp cell].
作者信息
Jiang Hong-wei, Ling Jun-qi, Zeng Jin-feng
机构信息
Department of Cariology and Endodontology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Jul;43(7):429-30.
OBJECTIVE
To investigated the effect of Escherichia coli (Ec) LPS on alkaline phosphatase (ALP) activity and expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) genes in vitro differentiation human dental pulp cell.
METHODS
Odontoblast-like cells were cultured, cells exposed to Ec LPS for 12 h, total RNA was isolated and DSPP, OCN transcripts were examined by real-time RT-PCR. ALP kit were used to assessed the changes of ALP activity.
RESULTS
Real-time RT-PCR analysis indicated that Ec LPS induced about a 3.6-fold decrease for DSPP gene and a 1.6-fold decrease for OCN gene in odontoblast-like cells as compared with controls. At the same time, cells treated with LPS could depress ALP activity from (1156.10 +/- 100.60) pmol x h(-1) x ng(-1) down to (884.80 +/- 26.72) pmol x h(-1) x ng(-1).
CONCLUSIONS
These results indicate that exposure of odontoblast-like cells to LPS can alter cells function by downregulating cell markers of odontoblastic activity.
目的
研究大肠杆菌脂多糖（Ec LPS）对体外分化的人牙髓细胞碱性磷酸酶（ALP）活性以及牙本质涎磷蛋白（DSPP）和骨钙素（OCN）基因表达的影响。
方法
培养成牙本质细胞样细胞，将细胞暴露于Ec LPS 12小时，提取总RNA，通过实时逆转录聚合酶链反应（RT-PCR）检测DSPP、OCN转录本。使用ALP试剂盒评估ALP活性的变化。
结果
实时RT-PCR分析表明，与对照组相比，Ec LPS使成牙本质细胞样细胞中DSPP基因下降约3.6倍，OCN基因下降1.6倍。同时，用LPS处理的细胞可使ALP活性从（1156.10±100.60）pmol·h⁻¹·ng⁻¹降至（884.80±26.72）pmol·h⁻¹·ng⁻¹。
结论
这些结果表明，成牙本质细胞样细胞暴露于LPS可通过下调成牙本质细胞活性的细胞标志物来改变细胞功能。
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