人牙髓细胞在胶原蛋白、明胶和壳聚糖支架上的不同分化特性。

Distinct differentiation properties of human dental pulp cells on collagen, gelatin, and chitosan scaffolds.

作者信息

Kim Na Ryoung, Lee Dong Hee, Chung Pill-Hoon, Yang Hyeong-Cheol

机构信息

Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul Korea.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Nov;108(5):e94-100. doi: 10.1016/j.tripleo.2009.07.031.

DOI:10.1016/j.tripleo.2009.07.031

PMID:19836718

Abstract

OBJECTIVE

The growth and differentiation properties of human dental pulp cells (HDPC) were investigated on a variety of natural scaffolds, including 2 types of collagen, gelatin, and chitosan.

STUDY DESIGN

Cell attachment and growth rates of HDPC on collagen (type I and type III), gelatin, and chitosan were observed. Alkaline phosphatase (ALP) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC on each scaffold were assessed.

RESULTS

Dental pulp cells attached and proliferated rapidly on collagen and gelatin, but chitosan did not properly support cell growth. The cells plated on gelatin exhibited high ALP activity, but not as high as cells plated on collagen. The expression peak of osteocalcin (OCN) mRNA from cells grown on collagen was found earlier and followed by dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP-1) mRNA expression. In cells grown on gelatin, however, OCN mRNA transcripts appeared at a later period of culture with no increase in DSPP or DMP-1 mRNA. Intensely mineralized extracellular matrix was seen in cells grown on collagen, but gelatin did not allow enough mineralization of cells in differentiation-inducing media.

CONCLUSION

Collagen supported proliferation and differentiation of HDPC, and the expression of DSPP and DMP-1 mRNA was reduced on gelatin.

摘要

目的

研究人牙髓细胞（HDPC）在多种天然支架上的生长和分化特性，这些支架包括2种胶原蛋白、明胶和壳聚糖。

研究设计

观察HDPC在胶原蛋白（I型和III型）、明胶和壳聚糖上的细胞附着和生长速率。评估每种支架上HDPC的碱性磷酸酶（ALP）活性、分化相关基因的mRNA表达以及矿化情况。

结果

牙髓细胞在胶原蛋白和明胶上附着并快速增殖，但壳聚糖不能很好地支持细胞生长。接种在明胶上的细胞表现出较高的ALP活性，但不如接种在胶原蛋白上的细胞高。在胶原蛋白上生长的细胞中，骨钙素（OCN）mRNA的表达峰值出现较早，随后是牙本质涎磷蛋白（DSPP）和牙本质基质蛋白1（DMP-1）mRNA的表达。然而，在明胶上生长的细胞中，OCN mRNA转录本在培养后期出现，且DSPP或DMP-1 mRNA没有增加。在胶原蛋白上生长的细胞中可见强烈矿化的细胞外基质，但明胶在诱导分化培养基中不能使细胞充分矿化。