通过凝胶双扩散和“夹心”酶联免疫吸附测定法检测系统性红斑狼疮、亚急性皮肤型红斑狼疮及干燥综合征中的抗Ro（SSA）抗体。

Detection of anti-Ro(SSA) antibodies by gel double diffusion and a 'sandwich' ELISA in systemic and subacute cutaneous lupus erythematosus and Sjögren's syndrome.

作者信息

Provost T T, Levin L S, Watson R M, Mayo M, Ratrie H

机构信息

Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

出版信息

J Autoimmun. 1991 Feb;4(1):87-96. doi: 10.1016/0896-8411(91)90009-2.

DOI:10.1016/0896-8411(91)90009-2

PMID:1903247

Abstract

A newly described Ro 'sandwich' ELISA was compared to the gel double diffusion technique to detect anti-Ro(SSA) antibodies in Sjögren's syndrome, systemic and subacute cutaneous lupus erythematosus patients. This study demonstrates that the ELISA assay increased the frequency of detection of anti-Ro(SSA) antibodies in these well defined connective tissue disease patients by approximately 5-10% compared to the gel double diffusion anti-Ro(SSA) antibody assay. The study also confirms that some patients make anti-Ro(SSA) antibodies directed solely at unique human Ro(SSA) antigen epitopes. We also detected the existence of a significant Sjögren's syndrome patient population failing to make significant anti-Ro(SSA) antibodies. We conclude from our study that the gel double-diffusion technique employing human spleen extract as a source of the Ro(SSA) antigen is, at present, the most cost-effective test to detect anti-Ro(SSA) antibodies.

摘要

将一种新描述的Ro“夹心”酶联免疫吸附测定（ELISA）与凝胶双向扩散技术进行比较，以检测干燥综合征、系统性红斑狼疮和亚急性皮肤型红斑狼疮患者体内的抗Ro（SSA）抗体。本研究表明，与凝胶双向扩散抗Ro（SSA）抗体检测法相比，ELISA检测法使这些明确诊断的结缔组织病患者体内抗Ro（SSA）抗体的检测频率提高了约5%-10%。该研究还证实，一些患者产生的抗Ro（SSA）抗体仅针对独特的人类Ro（SSA）抗原表位。我们还检测到有相当一部分干燥综合征患者未产生显著的抗Ro（SSA）抗体。我们从研究中得出结论，目前，采用人脾提取物作为Ro（SSA）抗原来源的凝胶双向扩散技术是检测抗Ro（SSA）抗体最具成本效益的方法。