Walker Louise A, Maccallum Donna M, Bertram Gwyneth, Gow Neil A R, Odds Frank C, Brown Alistair J P
Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.
Fungal Genet Biol. 2009 Feb;46(2):210-9. doi: 10.1016/j.fgb.2008.10.012. Epub 2008 Nov 6.
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.
对微生物病原体针对其哺乳动物宿主的分子反应进行全局分析是一项重大挑战。迄今为止，针对源自感染组织的白色念珠菌细胞开展的微阵列研究很少。在本研究中，我们检测了兔肾感染中白色念珠菌SC5314的转录组。与在RPMI 1640中生长的对照细胞相比，参与黏附、应激适应和替代碳源同化的基因在这些细胞中上调，而参与形态发生、发酵和翻译的基因则下调。当我们比较同基因的致病白色念珠菌菌株NGY152和SC5314时，它们在肾脏感染期间的转录组之间几乎没有重叠。这表明在感染过程中观察到的许多基因调控对毒力并非必不可少。事实上，我们观察到在肾脏感染期间受到调控且已经过毒力测试的那些基因的转录组和表型之间相关性较差。
