Lau Susanna K P, Lee Leo C K, Fan Rachel Y Y, Teng Jade L L, Tse Cindy W S, Woo Patrick C Y, Yuen Kwok-Yung
State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.
Int J Food Microbiol. 2009 Jan 31;129(1):78-82. doi: 10.1016/j.ijfoodmicro.2008.10.021. Epub 2008 Oct 31.
Laribacter hongkongensis is a recently discovered novel bacterium associated with community-acquired gastroenteritis. Although the bacterium has been isolated from freshwater fish and natural freshwater environments, it is not known if other freshwater animals could also be a source of L. hongkongensis. In a surveillance study on freshwater food animals (other than fish) in Hong Kong, L. hongkongensis was isolated from eight of 10 Chinese tiger frogs (Hoplobatrachus chinensis), a widespread frog species commonly consumed in China and southeast Asia. The large intestine was the site with the highest recovery rate, followed by the small intestine and stomach. None of the 30 Malaysian prawns, 20 pieces of sand shrimp, 20 Chinese mystery snails or 10 Chinese soft-shelled turtles was found to harbor the bacterium. Among the eight positive frogs, a total of 26 isolates of L. hongkongensis, confirmed by phenotypic tests and PCR, were obtained. As with human, freshwater fish and natural water isolates, a heterogeneous population of L. hongkongensis in frogs was identified by pulsed-field gel electrophoresis, with 6 different patterns among the 26 isolates and a single frog often carrying different strains. The present report represents the first to describe the isolation of L. hongkongensis from amphibians. The high isolation rate and genetic heterogeneity of L. hongkongensis among the Chinese tiger frogs suggested that these animals are also natural reservoir for the bacterium. Caution should be exercised in handling and cooking these frogs.
香港拉氏杆菌是一种最近发现的与社区获得性肠胃炎相关的新型细菌。尽管该细菌已从淡水鱼和天然淡水环境中分离出来，但尚不清楚其他淡水动物是否也可能是香港拉氏杆菌的来源。在一项对香港淡水食用动物（鱼类除外）的监测研究中，从10只中国虎纹蛙（虎纹蛙）中的8只分离出了香港拉氏杆菌，虎纹蛙是一种在中国和东南亚普遍食用的广泛分布的蛙类。大肠是检出率最高的部位，其次是小肠和胃。在30只马来西亚对虾、20只沙虾、20只中国圆田螺或10只中华软壳龟中均未发现携带该细菌。在8只阳性蛙中，通过表型试验和PCR共获得了26株经确认的香港拉氏杆菌分离株。与人类、淡水鱼和天然水分离株一样，通过脉冲场凝胶电泳鉴定出蛙体内香港拉氏杆菌群体具有异质性，26株分离株中有6种不同模式，且单只蛙通常携带不同菌株。本报告首次描述了从两栖动物中分离出香港拉氏杆菌。中国虎纹蛙中香港拉氏杆菌的高分离率和基因异质性表明，这些动物也是该细菌的天然宿主。处理和烹饪这些青蛙时应谨慎。
