Poole K, Neshat S, Heinrichs D
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
FEMS Microbiol Lett. 1991 Feb;62(1):1-5.
Reduced expression of an iron-regulated outer membrane protein (IROMP) of approximate molecular mass 90,000 was observed in Pseudomonas aeruginosa concomitant with a loss of pyoverdine production in a wild type strain grown at 43 degrees C and in a mutant deficient in pyoverdine production. Consistent with an implied role in pyoverdine-mediated iron transport a mutant lacking the 90 kDa protein transported barely detectable levels of ferri-pyoverdine. Interestingly, the mutant still exhibited pyoverdine-dependent growth in an iron-deficient medium containing the synthetic iron chelator ethylene diamine-di(omicron-hydroxyphenol acetic acid) (EDDHA) suggesting that a second uptake system for ferri-pyoverdine may exist in P. aeruginosa.
在铜绿假单胞菌中观察到，一种分子量约为90,000的铁调节外膜蛋白（IROMP）表达降低，同时在43摄氏度下生长的野生型菌株以及缺乏绿脓菌素产生的突变体中，绿脓菌素的产生也有所减少。与绿脓菌素介导的铁转运中所暗示的作用一致，缺乏90 kDa蛋白的突变体转运的铁-绿脓菌素水平几乎检测不到。有趣的是，该突变体在含有合成铁螯合剂乙二胺-二（邻羟基苯乙酸）（EDDHA）的缺铁培养基中仍表现出绿脓菌素依赖性生长，这表明铜绿假单胞菌中可能存在铁-绿脓菌素的第二种摄取系统。
