Aotaki-Keen A E, Harvey A K, de Juan E, Hjelmeland L M
Department of Ophthalmology, University of California, Davis, Sacramento.
Invest Ophthalmol Vis Sci. 1991 May;32(6):1733-8.
Glial cells of the human retina participate in various pathologic processes characterized by cell migration, proliferation, and extracellular matrix production. To study these events in vitro, a procedure was developed to obtain primary cultures of human retinal glial cells. The cultures resulting from the processing of 130 globes contained cells with variable morphology including bipolar and multipolar or stellate cells. Most cells in the primary culture were labeled with antisera to the glial fibrillary acidic protein. The cultures were also examined with antibodies directed against factor VIII-related antigen and muscle-specific actin to determine the presence of endothelial cells and pericytes. A variable contamination of cells staining for the latter was found in these cultures (usually less than 10%). Together, these data indicated that the primary cultures arose principally from glial cells of the human retina but did not precisely identify the cell of origin.
人类视网膜的神经胶质细胞参与各种以细胞迁移、增殖和细胞外基质产生为特征的病理过程。为了在体外研究这些事件，开发了一种获取人类视网膜神经胶质细胞原代培养物的方法。对130个眼球进行处理后得到的培养物中含有形态各异的细胞，包括双极细胞、多极细胞或星状细胞。原代培养中的大多数细胞都被抗胶质纤维酸性蛋白的抗血清标记。还用针对因子VIII相关抗原和肌肉特异性肌动蛋白的抗体对培养物进行检测，以确定内皮细胞和周细胞的存在。在这些培养物中发现了对后者呈染色阳性的细胞的不同程度污染（通常少于10%）。这些数据共同表明，原代培养物主要来源于人类视网膜的神经胶质细胞，但并未精确鉴定出细胞的起源。
