Shlomai J, Asher Y, Becker Y
J Gen Virol. 1977 Feb;34(2):223-34. doi: 10.1099/0022-1317-34-2-223.
The synthesis of herpes simplex virus DNA in isolated nuclei under in vitro conditions was found to be dependent on the addition of ATP and an ATP generating system to the reaction mixture. In vitro DNA synthesis was stimulated and prolonged when p-hydroxymercuribenzoate was added to the isolated nuclei. Under these improved conditions virus DNA molecules which were initiated in vivo were completed in vitro, but most of the DNA molecules synthesized in vitro sedimented in sucrose gradients more slowly than herpes virion DNA. Denaturation of the in vitro labelled DNA molecules produced short single-stranded labelled DNA chains. Thus, under our improved in vitro conditions there was prolonged synthesis of DNA at a high rate, with the formation of both complete and incomplete virus DNA molecules.
发现在体外条件下，分离细胞核中单纯疱疹病毒DNA的合成依赖于向反应混合物中添加ATP和ATP生成系统。当向分离的细胞核中添加对羟基汞苯甲酸时，体外DNA合成受到刺激并延长。在这些改进的条件下，体内起始的病毒DNA分子在体外得以完成，但体外合成的大多数DNA分子在蔗糖梯度中的沉降速度比疱疹病毒粒子DNA慢。体外标记的DNA分子变性产生短的单链标记DNA链。因此，在我们改进的体外条件下，DNA以高速率进行延长合成，同时形成完整和不完整的病毒DNA分子。
