Chen Ian Y, Greve Joan M, Gheysens Olivier, Willmann Jürgen K, Rodriguez-Porcel Martin, Chu Pauline, Sheikh Ahmad Y, Faranesh Anthony Z, Paulmurugan Ramasamy, Yang Phillip C, Wu Joseph C, Gambhir Sanjiv S
Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA, USA.
Mol Imaging Biol. 2009 May-Jun;11(3):178-87. doi: 10.1007/s11307-008-0182-z. Epub 2008 Nov 25.
In this study, we compared firefly luciferase (Fluc) reporter gene and superparamagnetic iron oxide (Feridex) as cell markers for longitudinal monitoring of cardiomyoblast graft survival using optical bioluminescence imaging (BLI) and magnetic resonance imaging (MRI), respectively.
Rats (n = 31) underwent an intramyocardial injection of cardiomyoblasts (2 x 10(6)) labeled with Fluc, Feridex, or no marker (control) or an injection of Feridex alone (75 microg). Afterward, rats were serially imaged with BLI or MRI and killed at different time points for histological analysis.
BLI revealed a drastically different cell survival kinetics (half-life = 2.65 days over 6 days) than that revealed by MRI (half-life = 16.8 days over 80 days). Injection of Feridex alone led to prolonged tissue retention of Feridex (> or =16 days) and persistent MR signal (> or =42 days).
Fluc BLI reporter gene imaging is a more accurate gauge of transplanted cell survival as compared to MRI of Feridex-labeled cells.
在本研究中，我们分别比较了萤火虫荧光素酶（Fluc）报告基因和超顺磁性氧化铁（Feridex）作为细胞标记物，用于通过光学生物发光成像（BLI）和磁共振成像（MRI）对心肌母细胞移植存活情况进行纵向监测。
对31只大鼠进行心肌内注射，注射用Fluc、Feridex标记的心肌母细胞（2×10⁶）或不进行标记（对照），或者单独注射Feridex（75微克）。之后，对大鼠进行连续的BLI或MRI成像，并在不同时间点处死以进行组织学分析。
BLI显示的细胞存活动力学（6天内半衰期=2.65天）与MRI显示的（80天内半衰期=16.8天）截然不同。单独注射Feridex导致Feridex在组织中的保留时间延长（≥16天）以及持续的磁共振信号（≥42天）。
与对Feridex标记细胞进行MRI相比，Fluc BLI报告基因成像对移植细胞存活情况的评估更为准确。
