Foltz Warren D, Cunningham Charles H, Mutsaers Anthony J, Conolly Steven M, Stewart Duncan J, Dick Alexander J
Department of Cardiology, St. Michael's Hospital, Toronto, Ontario, Canada.
J Cardiovasc Magn Reson. 2006;8(6):817-23. doi: 10.1080/10976640600777819.
Positive-contrast methods can augment negative-contrast methods for CMR evaluation of transplanted cell fate in vivo. This study correlated positive-contrast signal characteristics in the rabbit hind-limb with xenograft cell number (rat fibroblasts bearing endocytosed 1 microm Bang's particles) at different stages of Gd-DTPA administration and 1 mm2 in-plane resolution (2 minute scans, 1.5 Tesla). Linear regressions modeled satisfactorily the cell number dependencies of signal-to-noise (SNR) and area-of-enhancement (R > 0.83), over approximately a 50-fold fluctuation in cell number to a lowest detected limit of 25k cells during Gd-DTPA administration. Gd-DTPA administration elevated the slopes of parameter regressions by four or more standard deviations.
阳性对比剂方法可增强阴性对比剂方法，用于体内移植细胞命运的心脏磁共振成像（CMR）评估。本研究将兔后肢的阳性对比剂信号特征与不同钆喷酸葡胺（Gd-DTPA）给药阶段的异种移植细胞数量（内吞1微米班氏颗粒的大鼠成纤维细胞）以及1平方毫米的平面分辨率（2分钟扫描，1.5特斯拉）相关联。线性回归模型令人满意地模拟了信噪比（SNR）和强化面积（R>0.83）与细胞数量的依赖关系，在Gd-DTPA给药期间，细胞数量波动约50倍，最低检测限为25000个细胞。Gd-DTPA给药使参数回归的斜率提高了四个或更多标准差。
