Beijing University of Chemical Technology, People's Republic of China.
Appl Biochem Biotechnol. 2010 Jan;160(2):523-31. doi: 10.1007/s12010-008-8386-x. Epub 2008 Nov 26.
Coenzyme Q10 (CoQ10) is a vitamin-like substance which plays a crucial role in the respiratory chain ranging from bacteria to humans and in the radical scavenging in human body. In this study, the full-length hmgR gene (encoding 3-hydroxy-3-methyl-glutaryl-CoA reductase, HMG-CoA reductase) was cloned and overexpressed in Schizosaccharomyces pombe. Using the pREPG yeast depressed under the thiamine as the control, CoQ10 contents increased up to 2.68 and 3.09 times when recombinant cells were incubated without and with arachidonic acid, respectively. It demonstrated that arachidonic acid could upregulate the activity of HMG-CoA reductase and that hmgR gene played a significant role in CoQ10 biosynthesis. So, it has an importance to be utilized for fermentation.
辅酶 Q10（CoQ10）是一种类似维生素的物质，在从细菌到人类的呼吸链中起着至关重要的作用，并在人体中清除自由基。在这项研究中，全长 hmgR 基因（编码 3-羟基-3-甲基戊二酰辅酶 A 还原酶，HMG-CoA 还原酶）被克隆并在酿酒酵母中过表达。使用受硫胺素抑制的 pREPG 酵母作为对照，当重组细胞在没有和有花生四烯酸的情况下培养时，CoQ10 的含量分别增加了 2.68 和 3.09 倍。这表明花生四烯酸可以上调 HMG-CoA 还原酶的活性，并且 hmgR 基因在 CoQ10 的生物合成中起着重要作用。因此，它在发酵中具有重要的利用价值。
