Moriyama Daisuke, Hosono Kouji, Fujii Makoto, Washida Motohisa, Nanba Hirokazu, Kaino Tomohiro, Kawamukai Makoto
a QOL division , Kaneka Corporation , Takasago , Japan.
Biosci Biotechnol Biochem. 2015;79(6):1026-33. doi: 10.1080/09168451.2015.1006573. Epub 2015 Feb 3.
Coenzyme Q10 (CoQ10) is essential for energy production and has become a popular supplement in recent years. In this study, CoQ10 productivity was improved in the fission yeast Schizosaccharomyces pombe. Ten CoQ biosynthetic genes were cloned and overexpressed in S. pombe. Strains expressing individual CoQ biosynthetic genes did not produce higher than a 10% increase in CoQ10 production. In addition, simultaneous expression of all ten coq genes did not result in yield improvements. Genes responsible for the biosynthesis of p-hydroxybenzoate and decaprenyl diphosphate, both of which are CoQ biosynthesis precursors, were also overexpressed. CoQ10 production was increased by overexpression of Eco_ubiC (encoding chorismate lyase), Eco_aroF(FBR) (encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase), or Sce_thmgr1 (encoding truncated HMG-CoA reductase). Furthermore, simultaneous expression of these precursor genes resulted in two fold increases in CoQ10 production.
辅酶Q10（CoQ10）对于能量产生至关重要，近年来已成为一种流行的补充剂。在本研究中，裂殖酵母粟酒裂殖酵母中的CoQ10产量得到了提高。克隆了十个CoQ生物合成基因并在粟酒裂殖酵母中过表达。表达单个CoQ生物合成基因的菌株CoQ10产量的增加不超过10%。此外，同时表达所有十个coq基因并未提高产量。对对羟基苯甲酸酯和癸异戊二烯基二磷酸（二者均为CoQ生物合成前体）生物合成负责的基因也进行了过表达。通过过表达Eco_ubiC（编码分支酸裂解酶）、Eco_aroF(FBR)（编码3-脱氧-D-阿拉伯庚糖酸7-磷酸合酶）或Sce_thmgr1（编码截短的HMG-CoA还原酶），CoQ10产量增加。此外，同时表达这些前体基因使CoQ10产量提高了两倍。
