从超感染的拉吉细胞中获取的爱泼斯坦-巴尔病毒DNA用于互补RNA杂交研究的适用性。
Suitability of Epstein-Barr virus DNA obtained from superinfected Raji cells for complementary RNA hybridization studies.
作者信息
Tanaka A
出版信息
J Gen Virol. 1977 Feb;34(2):375-9. doi: 10.1099/0022-1317-34-2-375.
A method was established to obtain a high yield of Epstein-Barr virus (EBV) DNA for nucleic acid hybridization studies on latent virus DNA in transformed cells. Superinfection of Raji cells with EBV concentrated from HRI cell cultures produced a 600-fold higher yield of EBV DNA than direct isolation of EBV from HRI cell cultures. The virus DNA thus prepared from Raji cells superinfected with EBV was radioisotopically and spectrophotometrically pure and served as a satisfactory template for the preparation of cRNA specific to EBV DNA.
建立了一种方法，用于获得高产的爱泼斯坦-巴尔病毒（EBV）DNA，以用于对转化细胞中潜伏病毒DNA进行核酸杂交研究。用从HRI细胞培养物中浓缩的EBV对Raji细胞进行超感染，所产生的EBV DNA产量比直接从HRI细胞培养物中分离EBV高出600倍。用EBV超感染Raji细胞后制备的病毒DNA在放射性同位素和分光光度法检测下是纯净的，并且可作为制备EBV DNA特异性cRNA的理想模板。
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