Stoerker J, Yajima Y, Glaser R
Int J Cancer. 1982 Oct 15;30(4):385-92. doi: 10.1002/ijc.2910300402.
Two human lymphoblastoid cell lines were established by the transformation of human cord-blood lymphocytes with transforming Epstein-Barr virus (EBV). One cell line (HLB-R1) was established with EBV obtained after the superinfection of Raji cells with HR-1 EBV and the other (HLB-Bl) was established from B95-8 EBV-infected human cord-blood lymphocytes. Both the HLB-R1 and HLB-B1 lines were susceptible to superinfection with HR-1 EBV. We found that EBV DNA was replicated in the superinfected cell lines and that transforming EBV was produced in both the HLB-B1 and HLB-R1 cells. The average titer of transforming EBV obtained in the HR-1 EBV superinfected HLB-B1 and HLB-R1 cell lines was 10(4) transforming units (TU)/ml, whereas the average titers of transforming EBV obtained by the superinfection of Raji cells was 10(1) TU/ml. Epstein-Barr virus capable of inducing early antigen (EA) in superinfected Raji cells (lytic virus) was not detected in any transforming virus preparation. Restriction enzyme digestion patterns of virus DNA isolated from HR-1 and B95-8 cells, as well as from superinfected cells, were compared. The EBV DNA that was replicated in the superinfected HLB-R1 and HLB-B1 cell lines showed a more complex pattern. Our data suggest that recombination between input HR-1 EBV DNA and latent cell-associated EBV DNA occurs. Presumably this recombination results in a change in the biological properties of the newly synthesized virus.
通过用转化型爱泼斯坦 - 巴尔病毒（EBV）转化人脐血淋巴细胞，建立了两个人类淋巴母细胞系。一个细胞系（HLB - R1）是用HR - 1 EBV超感染Raji细胞后获得的EBV建立的，另一个（HLB - B1）是从感染B95 - 8 EBV的人脐血淋巴细胞建立的。HLB - R1和HLB - B1细胞系都易受HR - 1 EBV的超感染。我们发现EBV DNA在超感染的细胞系中复制，并且在HLB - B1和HLB - R1细胞中都产生了转化型EBV。在HR - 1 EBV超感染的HLB - B1和HLB - R1细胞系中获得的转化型EBV的平均滴度为10⁴转化单位（TU）/ml，而通过Raji细胞超感染获得的转化型EBV的平均滴度为10¹ TU/ml。在任何转化病毒制剂中均未检测到能够在超感染的Raji细胞中诱导早期抗原（EA）的爱泼斯坦 - 巴尔病毒（裂解病毒）。比较了从HR - 1和B95 - 8细胞以及超感染细胞中分离的病毒DNA的限制性酶切图谱。在超感染的HLB - R1和HLB - B1细胞系中复制的EBV DNA显示出更复杂的图谱。我们的数据表明输入的HR - 1 EBV DNA与潜伏的细胞相关EBV DNA之间发生了重组。据推测，这种重组导致新合成病毒的生物学特性发生变化。
