[Notch1和Notch2在星形细胞瘤和髓母细胞瘤中的差异表达]

[Differential expression of Notch1 and Notch2 in astrocytoma and medulloblastoma].

作者信息

Xu Peng, Pu Pei-Yu, Kang Chun-Sheng, Jia Zhi-Fan, Zhou Xuan, Wang Guang-Xiu

机构信息

Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-oncology, Tianjin Neurological Institute, Tianjin 300052, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2008 Jul;37(7):450-3.

PMID:19035115

Abstract

OBJECTIVE

To detect the differential expression of Notch1 and Notch2 in human astrocytoma and medulloblastoma; and to study the role of Notch1 and Notch2 in the development of both tumors.

METHODS

Immunohistochemical staining (SP method) and Western blot analysis were used to detect Notch1 and Notch2 expression in tissue arrays and freshly resected samples of normal brain tissue, astrocytoma and medulloblastoma.

RESULTS

Notch1 and Notch2 were negative in normal human brain tissue. Notch1 was highly expressed (total positive rate 80.0%, 48/60) while Notch2 was not detected in grade IV astrocytomas and sporadically observed in lower grade astrocytomas (total positive rate 10.0%, 6/60). The percentage of positive tumor cells and expression level of Notch1 increased with higher histologic grade (r = 0.859, P < 0.05). On the other hand, overexpression of Notch2 was detected in medulloblastoma (9/10) in contrast with lower expression of Notch1 (2/10).

CONCLUSIONS

Notch1 and Notch2 show differential expression in astrocytoma and medulloblastoma. This may be related to their different functional activities during the process of brain development.

摘要

目的

检测Notch1和Notch2在人星形细胞瘤和髓母细胞瘤中的差异表达；并研究Notch1和Notch2在这两种肿瘤发生发展中的作用。

方法

采用免疫组织化学染色（SP法）和蛋白质印迹分析检测正常脑组织、星形细胞瘤和髓母细胞瘤组织芯片及新鲜切除样本中Notch1和Notch2的表达。

结果

Notch1和Notch2在正常脑组织中呈阴性。Notch1在IV级星形细胞瘤中高表达（总阳性率80.0%，48/60），而在低级别星形细胞瘤中未检测到Notch2表达，仅偶有发现（总阳性率10.0%，6/60）。Notch1的阳性肿瘤细胞百分比和表达水平随组织学分级升高而增加（r = 0.859，P < 0.05）。另一方面，髓母细胞瘤中检测到Notch2过表达（9/10），而Notch1表达较低（2/10）。