Wu Ying, Li Meng-Tao, Zhao Yan, Feng Sheng, Su Li, Dong Yi
Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Nei Ke Za Zhi. 2008 Jul;47(7):563-5.
To detect anti-M3 receptor antibodies in primary Sjögren's syndrome (pSS) patients and to explore its association with clinical manifestations.
Anti-M3 antibodies were tested in 70 patients with pSS , 50 patients with systemic lupus erythematosus (SLE) and 76 normal controls with ELISA and Western blot. The correlation between anti-M3 antibodies and other clinical manifestations was analyzed.
(1) The positive rate of anti-M3 antibodies in pSS was 47.14% using ELISA and 60.00% using Western blot in SLE 4.00% using ELISA and 12.00% using Western blot and in normal controls 14.47% using ELISA and 15.79% using Western blot. (2) The incidence of schirmer test, tear break-up time (BUT), whole saliva flow rate, punctate epithelial erosions (PEE) on the corneas and external eye examination were not significantly different between the anti-M3 positive and negative groups. (3) The incidences of IgG, rheumatoid factor, SSB and fluorescence index (FI) > 3 were higher in the positive group than in the negative group using ELISA.
The positive rate of anti-M3 antibodies is higher in pSS than in SLE and normal controls and in some degree it has correlation with the lesion in salivary gland.
检测原发性干燥综合征（pSS）患者抗M3受体抗体，并探讨其与临床表现的相关性。
采用酶联免疫吸附测定（ELISA）和蛋白质印迹法检测70例pSS患者、50例系统性红斑狼疮（SLE）患者及76例正常对照者的抗M3抗体。分析抗M3抗体与其他临床表现之间的相关性。
（1）ELISA法检测pSS患者抗M3抗体阳性率为47.14%，蛋白质印迹法为60.00%；SLE患者ELISA法为4.00%，蛋白质印迹法为12.00%；正常对照者ELISA法为14.47%，蛋白质印迹法为15.79%。（2）抗M3抗体阳性组与阴性组的泪液分泌试验、泪膜破裂时间（BUT）、全唾液流速、角膜点状上皮糜烂（PEE）及眼部外部检查的发生率差异无统计学意义。（3）ELISA法检测显示，阳性组的免疫球蛋白G、类风湿因子、SSB及荧光指数（FI）>3的发生率高于阴性组。
pSS患者抗M3抗体阳性率高于SLE患者及正常对照者，且在一定程度上与唾液腺病变相关。
