Zhang Yan-Jun, Xu Dan-Ge, Fang Ye-Zhen, Gong Pu, Zhu Min, Bao Fang-Zhen
Zhejiang Province Center for Disease Control and Prevention, Hangzhou 310009, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Jul;42(7):499-501.
To investigate the contamination of Staphylococcus aureus isolates in ice cream by phenotypic typing and molecular typing.
The Staphylococcus aureus isolates were separated from ice cream, filler, cutter, salves and material. The separated isolates were characterized by drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E, G-J) genes and pulsed-field gel electrophoresis (PFGE) types.
Two Staphylococcus aureus isolates were separated, one from ice cream, another from cutter. Their characteristics of drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E,G-J) genes and PFGE type were the same.
The two Staphylococcus aureus isolates were the same clone. The contaminated Staphylococcus aureus isolates could be traced to the contaminated cutters.
通过表型分型和分子分型研究冰淇淋中金黄色葡萄球菌分离株的污染情况。
从冰淇淋、灌装机、切割机、软膏和原料中分离金黄色葡萄球菌分离株。对分离出的分离株进行耐药性、葡萄球菌肠毒素（SEA-E）、SE（A-E，G-J）基因和脉冲场凝胶电泳（PFGE）分型鉴定。
分离出两株金黄色葡萄球菌，一株来自冰淇淋，另一株来自切割机。它们的耐药性、葡萄球菌肠毒素（SEA-E）、SE（A-E，G-J）基因和PFGE分型特征相同。
这两株金黄色葡萄球菌分离株为同一克隆。污染的金黄色葡萄球菌分离株可追溯到受污染的切割机。
