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[工业生产的冰淇淋。金黄色葡萄球菌的发生率及产肠毒素能力]

[Ice cream of industrial manufacture. Incidence of Staphylococcus aureus and enterotoxigenicity].

作者信息

Rivas M, Cordal de Bobbi M E, Raffo Palma M, Moro A A

机构信息

Instituto Nacional de Farmacología y Bromatología, Buenos Aires, Argentina.

出版信息

Rev Argent Microbiol. 1984;16(4):225-8.

PMID:6336368
Abstract

A total of 70 samples of ice-creams, milk, milk cream and water or fruit based were obtained from 7 different industrial producers. Of these samples, 14.3% were positive for Staphylococcus aureus in 1 g: 97.2% showed less than 10 St. aureus in 1 g, 1.4% between 101-1,000 and 1.4% between 1,001-10,000. In 21 strains enterotoxigenicity, was investigated, as well as the response to different biochemical tests and the susceptibility to antibiotics: 9.5% of the strains were toxigenic (2 strains), 1 produced enterotoxin A and 1 enterotoxin B. There was no difference between toxigenic and nontoxigenic strains when the biochemical tests were performed (catalase test, coagulase test, thermostable nuclease production, anaerobic utilization of glucose and mannitol, gelatin and casein hydrolysis, production of hemolysins and pigments). All the strains showed a wide susceptibility when they were tested with different antibiotics. Our results confirm the limit introduced to the article 1078 of the Argentine Food Code for ice-creams of industrial production (100 per g) but there is no specification of the count method to be used.

摘要

从7家不同的工业生产商处共采集了70份冰淇淋、牛奶、奶油以及水基或水果基样本。在这些样本中,每克样本金黄色葡萄球菌检测呈阳性的占14.3%:97.2%的样本每克金黄色葡萄球菌含量低于10个,1.4%的样本每克含量在101 - 1000个之间,1.4%的样本每克含量在1001 - 10000个之间。对21株菌株进行了产肠毒素能力的研究,以及不同生化试验的反应和对抗生素的敏感性研究:9.5%的菌株具有产肠毒素能力(2株),1株产生肠毒素A,1株产生肠毒素B。进行生化试验(过氧化氢酶试验、凝固酶试验、耐热核酸酶产生试验、葡萄糖和甘露醇的厌氧利用试验、明胶和酪蛋白水解试验、溶血素和色素产生试验)时,产毒菌株和无毒菌株之间没有差异。用不同抗生素检测时,所有菌株都表现出广泛的敏感性。我们的结果证实了阿根廷食品法典第1078条对工业生产冰淇淋规定的限量(每克100个),但未规定所使用的计数方法。

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